Radioimmunological detection of anti-transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow-up
M. BONAMICO*, R. NENNA*, R. P. L. LUPARIA*, C. PERRICONE*, M. MONTUORI*, F. LUCANTONI*, A. CASTRONOVO*, S. MURA*, A. TURCHETTI*, P. STRAPPINI* & C. TIBERTI†
*Department of Paediatrics and †Department of Clinical Science, "Sapienza" University of Rome, Rome, Italy.
Correspondence to Prof. M. Bonamico, Department of Paediatrics, "Sapienza" University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.
E-mail: margherita.bonamico@uniroma1.it
Copyright Journal compilation © 2008 Blackwell Publishing Ltd
ABSTRACT
Background Serum radioimmunoassay (RIA) tissue transglutaminase autoantibodies (tTG-Abs) proved to be a sensitive test also during coeliac disease (CD) follow-up. We demonstrated that RIA tTG-Abs could be detected in human saliva.
Aim To evaluate salivary RIA tTG-Abs in coeliac children on gluten-free diet (GFD).
Methods Saliva and serum samples from 109 coeliac children were evaluated at diagnosis (group 1: 71 females, median age 9.4 years) and 58 of them on GFD: 36 after 3–6 months (group 2a), 34 at 9 months or more (group 2b). Two gender- and age-matched control groups: 89 gastroenterological patients (group 3) and 49 healthy subjects (group 4) participated in the study. Saliva and serum tTG-Abs were detected by RIA and compared with serum tTG-Abs ELISA and IgA anti-endomysium antibodies (EMA).
Results Salivary RIA tTG-Abs were found in 94.5%, 66.7% and 50.0% of groups 1, 2a and 2b CD patients and in 98.2%, 72.2% and 50.0% of corresponding serum samples, respectively. tTG-Abs decreased with GFD progression and a correlation was found between saliva and serum titres (r = 0.75, P = 0.0001). During the CD follow-up, salivary and serum RIA sensitivities were comparable, and higher with respect to EMA and ELISA.
Conclusions This study demonstrates that it is possible to detect salivary tTG-Abs with high sensitivity not only at CD diagnosis, but also during GFD.
Gluten-free, ROCK, Raising our celiac kids, organic,
celiac,children, diet, bellingham, naturopathic,
naturopath, twins, acupuncture, osteoporosis, gluten
enteropathy, gluten intolerance, Whole foods, non-celiac gluten enteropathy
M. BONAMICO*, R. NENNA*, R. P. L. LUPARIA*, C. PERRICONE*, M. MONTUORI*, F. LUCANTONI*, A. CASTRONOVO*, S. MURA*, A. TURCHETTI*, P. STRAPPINI* & C. TIBERTI†
*Department of Paediatrics and †Department of Clinical Science, "Sapienza" University of Rome, Rome, Italy.
Correspondence to Prof. M. Bonamico, Department of Paediatrics, "Sapienza" University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.
E-mail: margherita.bonamico@uniroma1.it
Copyright Journal compilation © 2008 Blackwell Publishing Ltd
ABSTRACT
Background Serum radioimmunoassay (RIA) tissue transglutaminase autoantibodies (tTG-Abs) proved to be a sensitive test also during coeliac disease (CD) follow-up. We demonstrated that RIA tTG-Abs could be detected in human saliva.
Aim To evaluate salivary RIA tTG-Abs in coeliac children on gluten-free diet (GFD).
Methods Saliva and serum samples from 109 coeliac children were evaluated at diagnosis (group 1: 71 females, median age 9.4 years) and 58 of them on GFD: 36 after 3–6 months (group 2a), 34 at 9 months or more (group 2b). Two gender- and age-matched control groups: 89 gastroenterological patients (group 3) and 49 healthy subjects (group 4) participated in the study. Saliva and serum tTG-Abs were detected by RIA and compared with serum tTG-Abs ELISA and IgA anti-endomysium antibodies (EMA).
Results Salivary RIA tTG-Abs were found in 94.5%, 66.7% and 50.0% of groups 1, 2a and 2b CD patients and in 98.2%, 72.2% and 50.0% of corresponding serum samples, respectively. tTG-Abs decreased with GFD progression and a correlation was found between saliva and serum titres (r = 0.75, P = 0.0001). During the CD follow-up, salivary and serum RIA sensitivities were comparable, and higher with respect to EMA and ELISA.
Conclusions This study demonstrates that it is possible to detect salivary tTG-Abs with high sensitivity not only at CD diagnosis, but also during GFD.